To investigate how norovirus breaches the intestinal epithelial lining to cause acute gastroenteritis. I utilized techniques I had never used before, from in situ hybridization to track virus movement across different timepoints in the intestinal tissue to confocal microscopy to visualize our results. My main responsibility was to conduct RNAScope on prepared intestinal slides from neonates across different time points to observe and explain when the virus crosses the intestinal barrier to cause infection. I was primarily counting the number of red dots I saw -- these indicate viral RNA. To determine what intestinal "mechanism" the virus was using to breach the intestinal barrier, I used fluorescent antibodies to identify structures of interest that we believed the virus might be using to get past the epithelial barrier to infect underlying immune cells. I conducted confocal microscopy on these samples. I also performed a number of organ harvests from the GI tract of neonatal mice to be used for the RNAScope slides.